杨秀惠,王志杰,周勇,张红榕,陈致飞,张苏晗,郑凝旋,李东

• 1:福建省疾病预防控制中心福建省人兽共患病重点实验室
• 2:福建医科大学公共卫生学院教学基地

摘要(Abstract)：

目的了解福建省II型脊髓灰质炎(脊灰)疫苗相关株病毒(VAPV2)变异特征,为证实阻断VAPV2传播提供依据。方法采用RT-PCR扩增VAPV的VP1全长和部分3D区基因序列,统计分析病毒核苷酸(nt)和氨基酸(aa)变异情况。结果 2000-2015年福建省从5 497份粪便标本中分离到219株脊灰病毒,1株为疫苗衍生脊灰病毒(VDPV),218株为VAPV,其中VAPV2占37.16%。与Sabin2株相比,47株VAPV2中VP1变异位点>5个2株(4.26%),≤2个36株(76.60%);共涉及45个位点,其中nt2909为突变热点,导致VP1的aa143位点从非极性疏水性转换成极性亲水性。13株VAPV在3D区出现重组。结论福建省大部分VAPV2变异少,VP1的aa143位点为突变热点。

关键词(KeyWords)： 脊髓灰质炎疫苗相关株病毒;病毒变异

Abstract:

Keywords:

基金项目(Foundation): 福建省卫生系统中青年骨干人才培养项目(2013-ZQN-ZD-10)

作者(Author): 杨秀惠,王志杰,周勇,张红榕,陈致飞,张苏晗,郑凝旋,李东

DOI: 10.19914/j.cjvi.2017.03.005

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